Trypsin EDTA

(Expressed in Pichia pastoris)

Standard for High-Performance Cell Detachment

100% Animal Origin Free 

CAS No: 9002-07-7  

Product Overview

Maximize cell viability and regulatory safety with our Recombinant Trypsin EDTA, a high-purity, animal-origin-free (AOF) solution engineered for the modern laboratory. Developed specifically for adherent mammalian cell lines—including VERO, CHO, HEK 293, MRC-5, and MDCK—this genetically engineered enzyme offers a precise, stable, and risk-free alternative to traditional porcine-derived trypsin. 

Why Choose Recombinant Over Porcine? 

Traditional trypsin harvested from porcine pancreas carries inherent risks of viral contamination and batch-to-batch variability. Our recombinant version, expressed in Pichia pastoris, eliminates these concerns while providing a 100% Animal-Origin-Free (AOF) environment. 

 The Power of EDTA Integration 

Our 1X formulation is enriched with EDTA to enhance the “trypsinization” process. By effectively sequestering calcium (Ca2+) and magnesium (Mg2+) ions—the “glue” that holds cells to culture surfaces—EDTA allows for faster and gentler detachment at lower enzyme concentrations. 

Applications

  • Advanced Cell Culture & Subculturing
  • Serum-Free Workflows
  • Tissue Dissociation

Technical Specifications

Formulation: 1x, without Calcium & Magnesium

Purity: ≥ 95% by SDS – PAGE

Storage: Within specification range for 24 months –20°C to -80°C

Regulatory: GMP-Compliant / AOF / Xeno-Free 

Related Products

Trypsin

Insulin & Vaccine Production

Carboxypeptidase B

Insulin & Monoclonal Antibody Production

Human Albumin

Cell therapy, Vaccines, Serum-free Media

Endonuclease

Viscosity Reduction, Insulin Manufacturing.

N Glycosidase F

Deglycosylation of Glycoproteins, Glycan Structure & Profiling Studies

Enterokinase

Recombinant Protein Processing & Purification.

KEX2 Protease

Proteolytic Cleavage of Dibasic Peptide Bonds

Scroll to Top