Endonuclease

(Expressed in E.Coli)

Recombinant Endonuclease

CAS No: 9025-65-4

Product Overview

Optimize your nucleic acid workflows with our Endonuclease, the gold standard for selective degradation of single-stranded DNA (ssDNA). Engineered for high stability and zero double-stranded activity, this Zinc-dependent endonuclease is a critical tool for researchers demanding the highest fidelity in cDNA cloning, mRNA synthesis, and transcript mapping. 

Why use Endonuclease? 

Unlike other standard extracts, our formulation is refined for the modern molecular biology suite, offering three distinct advantages: 

  • Absolute Strand Specificity: Unlike lower-grade endonucleases, our Endonuclease ensures the total preservation of double-stranded DNA (dsDNA) and DNA-RNA hybrids, even at high concentrations. 
  • Ultra-Low Non-Specific Activity: Rigorously tested to ensure the absence of contaminating proteases and double-strand-specific nucleases that can ruin precious samples. 

Applications

  • Viral clearance and virus-like particles purification in vaccine manufacturing 
  • Targeted degradation of interfering nucleic acid contaminants 
  • Advanced sample preparation for electrophoresis and chromatography  
  • Prevention of DNA-mediated cell aggregation (Cell Clumping) 

Technical Specifications

Molecular weight  : 30kDa

Purity: ≥ 99%

Formulated with: Glycerol, Tri-HCl, NaCl And MgCl2 

Specific Activity: ≥ 250u/µl and no detectable protease activity 

Available Strength: 100 kU, 150kU, 1MU and 5MU per vial

Storage: Within specification range for 24 months –20°C to -80°C

Regulatory: GMP-Compliant / AOF / Xeno-Free 

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