KEX2 Protease
(Expressed in Pichia pastoris)
100% Animal Origin Free
CAS No: 99676-46-7
Product Overview
Recombinant Kex2 Protease is a calcium-dependent serine protease engineered to mimic the high fidelity of the yeast Kexin-family endoprotease.
Designed to specifically recognize and cleave the carboxyl peptide bond of dibasic amino acids—primarily Lys-Arg (K-R) and Arg-Arg (R-R)—our Kex2 Protease is the essential tool for manufacturers seeking to produce bioactive proteins from complex fusion precursors.
Why Recombinant Kex2 Protease is the Industry Choice?
- Superior Substrate Specificity: Recombinant Kex2 Protease does not cleave at isolated basic residues, ensuring that your target protein remains intact while only the fusion tag or pro-peptide is removed.
- Animal-Free (AOF) Manufacturing: Our Kex2 is produced in a recombinant microbial system, making it completely free of animal-derived components, and ideal for clinical-grade therapeutic production.
- High Specific Activity: Optimized for rapid processing, allowing for low enzyme-to-substrate ratios that reduce costs and simplify downstream purification.
- Robust Performance: Retains high activity across a broad range of buffer conditions, specifically optimized for pH 7.0–9.0 in the presence of calcium ions.
Applications
- Site-Specific Tag Removal and pro-peptides from recombinant proteins to recover native protein structures.
- Specialized in the processing of secreted proteins, enzymes, and bioactive peptides in recombinant expression systems.
- A critical component for both research and large-scale industrial bioprocessing and downstream purification workflows.
- Reliable processive enzymatic cleavage for the synthesis and maturation of peptide-based therapeutics (e.g., Insulin, GLP-1).
- Ideal for the enzymatic digestion of proteins for high-resolution peptide mapping and amino acid sequencing studies.
- Used in the maturation of viral antigens expressed in recombinant systems to ensure correct folding and immunogenicity
Technical Specifications
Molecular weight : 70kDa
Purity: ≥ 95%, confirmed by stringent quality control test
Optimal pH: 4.5 – 5.5
Forms: Liquid and Lyophilized
Storage: Within specification range for 24 months –25°C to -15°C
Regulatory: GMP-Compliant / AOF
